technology Detailed by Investigators at China Pharmaceutical University -->
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Biotechnology. According to news reporting originating in Jiangsu, People's Republic of China, by NewsRx journalists, research stated, "Enantiomerically pure l-homophenylalanine (l-HPA) is a key building block for the synthesis of angiotensin-converting enzyme inhibitors and other chiral pharmaceuticals. Among the processes developed for the l-HPA production, biocatalytic synthesis employing phenylalanine dehydrogenase has been proven as the most promising route."
The news reporters obtained a quote from the research from China Pharmaceutical University, "However, similar to other dehydrogenase-catalyzed reactions, the viability of this process is markedly affected by insufficient substrate loading and high costs of the indispensable cofactors. In the present work, a highly efficient and economic biocatalytic process for l-HPA was established by coupling genetically modified phenylalanine dehydrogenase and formate dehydrogenase. Combination of fed-batch substrate addition and a continuous product removal greatly increased substrate loading and cofactor utilization. After systemic optimization, 40 g (0.22 mol) of keto acid substrate was transformed to l-HPA within 24 h and a total of 0.2 mM NAD(+) was reused effectively in eight cycles of fed-batch operation, consequently giving an average substrate concentration of 510 mM and a productivity of 84.1 g l(-1) day(-1) for l-HPA."
According to the news reporters, the research concluded: "The present study provides an efficient and feasible enzymatic process for the production of l-HPA and a general solution for the increase of substrate loading."
For more information on this research see: Enhancement of biocatalytic efficiency by increasing substrate loading: enzymatic preparation of l-homophenylalanine. Applied Microbiology and Biotechnology, 2013;97(19):8487-8494. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)
Our news correspondents report that additional information may be obtained by contacting J.L. Zhang, China Pharmaceutical University, State Key Lab Nat Med, Nanjing 210009, Jiangsu, People's Republic of China. Additional authors for this research include T.Y. Zhu, X.R. Wu and Y.J. Chen (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Jiangsu, Dehydrogenase, Enzymes and Coenzymes, People's Republic of China
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