By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Silicon Dioxide have been published. According to news originating from Fuzhou, People's Republic of China, by NewsRx correspondents, research stated, "A novel displacement-type quartz crystal microbalance (QCM) immunosensing strategy, based on glucose and its analogue dextran for concanavalin A (ConA) binding sites, was designed for ultrasensitive monitoring of small molecular biotoxins (brevetoxin B, PbTx-2, used as a model) with signal amplification on a graphene-functionalized sensing interface. To construct such a QCM immunosensing platform, phenoxy-functionalized dextran (DexP) was initially assembled onto the surface of graphene-coated QCM probe via the ?-stacking interaction, and ConA-labeled monoclonal mouse anti-PbTx-2 capture antibody was then immobilized on the DexP-modified probe by dextran-ConA binding."
Our news journalists obtained a quote from the research from Fuzhou University, "Gold nanoparticle heavily functionalized with glucoamylase and bovine serum albumin-PbTx-2 (PbTx-2-BSA) conjugate was employed as the trace tag. A competitive-type immunoassay format was adopted for the online monitoring of PbTx-2 between anti-PbTx-2 antibody immobilized on the QCM probe and PbTx-2-BSA labeled on the gold nanoparticle. Accompanying the gold nanoparticle, the carried glucoamylase could hydrolyze amylopectin in glucose. The produced glucose competed with dextran for ConA and displaced the ConA-streptavidin-anti-PbTx-2 complex from the QCM probe, resulting in the frequency change. Under optimal conditions, the frequency of the QCM immunosensor was indirectly proportional to the concentration of target PbTx-2 in the sample and exhibited a dynamic range from 1.0 pg·mL(-1) to 10 ng·mL(-1) with a detection limit (LOD) of 0.6 pg·mL(-1) at the 3Sblank level. Intra-and interassay coefficients of variation were below 7.5% and 9.5%, respectively."
According to the news editors, the research concluded: "In addition, the methodology was evaluated for analysis of PbTx-2 in 15 spiked seafood samples and showed good accordance between results obtained by the displacement-type QCM immunosensor and a commercialized enzyme-linked immunosorbent assay (ELISA) method."
For more information on this research see: Displacement-type quartz crystal microbalance immunosensing platform for ultrasensitive monitoring of small molecular toxins. Analytical Chemistry, 2013;85(14):6958-66. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
The news correspondents report that additional information may be obtained from D. Tang, Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Dept. of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, People's Taiwan. Additional authors for this research include B. Zhang, J. Tang, L. Hou and G. Chen (see also Silicon Dioxide).
Keywords for this news article include: Asia, Antibodies, Fuzhou, Quartz, Immunology, Blood Proteins, Nanotechnology, Immunoglobulins, Silicon Dioxide, Gold Nanoparticles, Emerging Technologies, People's Republic of China.
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