Spanish National Research Council (CSIC) Update Knowledge of Biodeterioration and Biodegradation -->
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biodeterioration and Biodegradation have been published. According to news reporting from Barcelona, Spain, by NewsRx journalists, research stated, "We investigated the decayed historical church window glasses of two Catalonian churches, both under Mediterranean climate. Glass surfaces were studied by scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD)."
The news correspondents obtained a quote from the research from Spanish National Research Council (CSIC), "Their chemical composition was determined by wavelength-dispersive spectrometry (WDS) microprobe analysis. The biodiversity was investigated by molecular methods: DNA extraction from glass, amplification by PCR targeting the 16S rRNA and ITS regions, and fingerprint analyses by denaturing gradient gel electrophoresis (DGGE). Clone libraries containing either PCR fragments of the bacterial 16S rDNA or the fungal ITS regions were screened by DGGE. Clone inserts were sequenced and compared with the EMBL database. Similarity values ranged from 89 to 100% to known bacteria and fungi. Biological activity in both sites was evidenced in the form of orange patinas, bio-pitting, and mineral precipitation. Analyses revealed complex bacterial communities consisting of members of the phyla Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. Fungi showed less diversity than bacteria, and species of the genera Cladosporium and Phoma were dominant. The detected Actinobacteria and fungi may be responsible for the observed bio-pitting phenomenon. Moreover, some of the detected bacteria are known for their mineral precipitation capabilities."
According to the news reporters, the research concluded: "Sequence results also showed similarities with bacteria commonly found on deteriorated stone monuments, supporting the idea that medieval stained glass biodeterioration in the Mediterranean area shows a pattern comparable to that on stone."
For more information on this research see: Microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two Catalonian churches. International Biodeterioration & Biodegradation, 2013;84():388-400. International Biodeterioration & Biodegradation can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; International Biodeterioration & Biodegradation - www.elsevier.com/wps/product/cws_home/405899)
Our news journalists report that additional information may be obtained by contacting G. Pinar, CSIC, Inst Ciencies Terra Jaume Almera, E-08028 Barcelona, Spain. Additional authors for this research include M. Garcia-Valles, D. Gimeno-Torrente, J.L. Fernandez-Turiel, J. Ettenauer and K. Sterflinger (see also Biodeterioration and Biodegradation).
Keywords for this news article include: Spain, Europe, Barcelona, Biodeterioration and Biodegradation
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