By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biological Factors have been published. According to news reporting out of Singapore, Singapore, by NewsRx editors, research stated, "The current mycotoxin safety concerns demand the availability of reliable and selective control systems to measure the content of mycotoxins present in daily food. To address this, we report a prospective analytical tool involving the application of a flow injection mass spectrometry (FI/MS) tandem artificial neural network (ANN) strategy to predict the amount of ochratoxin A and B (OTA and OTB) in beer."
Our news journalists obtained a quote from the research from Applied Sciences Group, "Triple stage mass spectrometry (MS3) aided by chromatographic separation was applied as a reference method for comparison. 0.1% formic acid and methanol were used to convert ochratoxins into their respective ions under negative MS polarity. For experiments involving MS3, ochratoxins were separated by reversed-phase liquid chromatography in a 6 min run, ionised using electrospray ionisation, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge ratios were used to perform quantitation in MS3 mode. For experiments involving FI/MS, no chromatographic separation was performed. Approximately 2% of the mass spectra was used for model construction. ANN models representing each ochratoxin were individually trained and validated using three sets of matrix-matched and matrix-free calibration curves at nine concentration levels of 2.5, 10, 25, 50, 100, 200, 300, 400 and 500 mu g/l. Quintuplicate analyses were made in FI/MS mode providing a total of 270 spectra for both OTA and OTB. Single measurement was made for each sample in MS3 mode. A root-mean-square error value of
According to the news editors, the research concluded: "The successful utilisation of FILMS without performing chromatographic separation implies the availability of a new analytical tool relevant to the field of mycotoxin analysis, possibly offering analyte specificity exceeding the capability of MS3 through chemometry."
For more information on this research see: Single laboratory method validation comparing MS3 with FI/MS fingerprinting, and quantitation strategies for the accurate determination of ochratoxins in beer. World Mycotoxin Journal, 2013;6(3):281-290. World Mycotoxin Journal can be contacted at: Wageningen Academic Publishers, PO Box 220, Wageningen, 6700 Ae, Netherlands (see also Biological Factors).
Our news journalists report that additional information may be obtained by contacting C.W. Lim, Hlth Sci Author, Appl Sci Grp, Food Safety Lab, Singapore 169078, Singapore.
Keywords for this news article include: Asia, Mycotoxins, Ochratoxins, Biological Factors
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