By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Analytical and Bioanalytical Chemistry are presented in a new report. According to news reporting originating in Munster, Germany, by NewsRx journalists, research stated, "A high analytical sensitivity in ultraviolet matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is only achieved if the laser wavelength corresponds to a high optical absorption of the matrix. Laser fluence and the physicochemical properties of the compounds, e.g., the proton affinity, also influence analytical sensitivity significantly."
The news reporters obtained a quote from the research from the University of Munster, "In combination, these parameters determine the amount of material ejected per laser pulse and the ion yield, i.e., the fraction of ionized biomolecules. Here, we recorded peptide ion signal intensities as a function of these parameters. Three cinnamic acid matrices were investigated: alpha-cyano-4-hydroxycinnamic acid, alpha-cyano-4-chlorocinnamic acid, and alpha-cyano-2,4-difluorocinnamic acid. In addition, 2,5-dihydroxybenzoic acid was used in comparison experiments. Ion signal intensities 'per laser shot' and integrated ion signal intensities were acquired over 900 consecutive laser pulses applied on distinct positions on the dried-droplet sample preparations. With respect to laser wavelength, the two standard MALDI wavelengths of 337/355 nm were investigated. Also, 305 or 320 nm was selected to account for the blue-shifted absorption profiles of the halogenated derivatives. Maximal peptide ion intensities were obtained if the laser wavelength fell within the peak of the absorption profile of the compound and for fluences two to three times the corresponding ion detection threshold."
According to the news reporters, the research concluded: "The results indicate ways for improving the analytical sensitivity in MALDI-MS, and in particular for MALDI-MS imaging applications where a limited amount of material is available per irradiated pixel."
For more information on this research see: Matching the laser wavelength to the absorption properties of matrices increases the ion yield in UV-MALDI mass spectrometry. Analytical and Bioanalytical Chemistry, 2013;405(22):6925-6932. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
Our news correspondents report that additional information may be obtained by contacting M. Wiegelmann, University of Munster, Inst Med Phys & Biophys, D-48149 Munster, Germany. Additional authors for this research include J. Soltwisch, T.W. Jaskolla and K. Dreisewerd (see also Analytical and Bioanalytical Chemistry).
Keywords for this news article include: Europe, Munster, Germany, Analytical and Bioanalytical Chemistry
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