By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Proteobacteria have been published. According to news originating from Columbia, Missouri, by NewsRx correspondents, research stated, "Water and wastewater filtration systems often house pathogenic bacteria, which. must be removed to ensure clean, safe water. Here, we determine the persistence of the model bacterium Pseudomonas aeruginosa in two types of filtration systems, and use P. aeruginosa bacteriophages to determine their ability to selectively remove P. aeruginosa."
Our news journalists obtained a quote from the research from the University of Missouri, "These systems used beds of either anthracite or granular activated carbon (GAC), which were operated at an empty bed contact time (EBCT) of 45 min. The clean bed filtration systems were loaded with an instantaneous dose of P. aeruginosa at a total cell number of 2.3 (+/- 0.1 [standard deviation]) x 10(7) cells. An immediate dose of P. aeruginosa phages (1 mL of phage stock at the concentration of 2.7 x 10(7) PFU (Plaque Forming Units)/mL) resulted in a reduction of 50% (+/- 9%) and >99.9% in the effluent P. aeruginosa concentrations in the clean anthracite and GAC filters, respectively. To further evaluate the effects of P. aeruginosa phages, synthetic stormwater was run through anthracite and GAC biofilters where mixed-culture biofilms were present. Eighty five days after an instantaneous dose of P. aeruginosa (2.3 x 10(7) cells per filter) on day 1, 7.5 (+/- 2.8) x 10(7) and 1.1 (+/- 0.5) x 10(7) P. aeruginosa cells/g filter media were detected in the top layer (close to the influent port) of the anthracite and GAC biofilters, respectively, demonstrating the growth and persistence of pathogenic bacteria in the biofilters. A subsequent 1-h dose of phages, at the concentration of 5.1 x 10(6) PFU/mL and flow rate of 1.6 mL/min, removed the P. aeruginosa inside the GAC biofilters and the anthracite biofilters by 70% (+/- 5%) and 56% (+/- 1%), respectively, with no P. aeruginosa detected in the effluent, while not affecting ammonia oxidation or the ammonia-oxidizing bacterial community inside the biofilters."
According to the news editors, the research concluded: "These results suggest that phage treatment can selectively remove pathogenic bacteria with minimal impact on beneficial organisms from attached growth systems for effluent quality improvement."
For more information on this research see: Application of bacteriophages to selectively remove Pseudomonas aeruginosa in water and wastewater filtration systems. Water Research, 2013;47(13):4507-4518. Water Research can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Water Research - www.elsevier.com/wps/product/cws_home/309)
The news correspondents report that additional information may be obtained from Y.Y. Zhang, University of Missouri, Dept. of Bioengn, Columbia, MO 65211, United States. Additional authors for this research include H.K. Hunt and Z.Q. Hu (see also Proteobacteria).
Keywords for this news article include: Viruses, Columbia, Missouri, United States, Bacteriophages, Pseudomonadaceae, Gammaproteobacteria, Gram-Negative Bacteria, Pseudomonas aeruginosa, North and Central America, Gram-Negative Aerobic Bacteria, Gram-Negative Aerobic Rods and Cocci
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