By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on RNA Research. According to news reporting originating in Ithaca, New York, by NewsRx journalists, research stated, "Antisense RNA transcription attenuators are a key component of the synthetic biology toolbox, with their ability to serve as building blocks for both signal integration logic circuits and transcriptional cascades. However, a central challenge to building more sophisticated RNA genetic circuitry is creating larger families of orthogonal attenuators that function independently of each other."
The news reporters obtained a quote from the research from Cornell University, "Here, we overcome this challenge by developing a modular strategy to create chimeric fusions between the engineered transcriptional attenuator from plasmid pT181 and natural antisense RNA translational regulators. Using in vivo gene expression assays in Escherichia coli, we demonstrate our ability to create chimeric attenuators by fusing sequences from five different translational regulators. Mutagenesis of these functional attenuators allowed us to create a total of 11 new chimeric attenutaors. A comprehensive orthogonality test of these culminated in a 7 x 7 matrix of mutually orthogonal regulators. A comparison between all chimeras tested led to design principles that will facilitate further engineering of orthogonal RNA transcription regulators, and may help elucidate general principles of non-coding RNA regulation."
According to the news reporters, the research concluded: "We anticipate that our strategy will accelerate the development of even larger families of orthogonal RNA transcription regulators, and thus create breakthroughs in our ability to construct increasingly sophisticated RNA genetic circuitry."
For more information on this research see: A modular strategy for engineering orthogonal chimeric RNA transcription regulators. Nucleic Acids Research, 2013;41(15):7577-7588. Nucleic Acids Research can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Nucleic Acids Research - nar.oxfordjournals.org)
Our news correspondents report that additional information may be obtained by contacting M.K. Takahashi, Cornell University, Sch Chem & Biomol Engn, Ithaca, NY 14853, United States (see also RNA Research).
Keywords for this news article include: Antisense Technology, Biotechnology, Ithaca, New York, Genetics, RNA Research, United States, Bioengineering, Medical Devices, RNA Transcription, North and Central America
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