By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Investigators publish new report on Biotechnology. According to news reporting originating in Chongqing, People's Republic of China, by NewsRx journalists, research stated, "To determine whether curcumin reverses the multidrug resistance of human colon cancer cells in vitro and in vivo. In a vincristine-resistant cell line of human colon cancer, the cell viability of curcumin-treated cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay."
The news reporters obtained a quote from the research from Chongqing Medical University, "Rhodamine123 efflux was evaluated to detect P-glycoprotein transporter activity, and expression of the multidrug resistance protein 1 and survivin genes was analyzed by reverse transcription polymerase chain reaction and western blotting. In addition, xenograft mouse tumors were grown and treated with curcumin. The morphology of the xenografts was investigated by hematoxylin-eosin staining. The in vivo expression of the multidrug resistance gene and P-glycoprotein and survivin genes and proteins was observed using reverse transcription-polymerase chain reaction and western blotting, respectively. Curcumin was not obviously toxic to the vincristine-resistant human colon cancer cells at concentrations less than 25 ?M, but the growth of cells was significantly inhibited. At concentrations greater than 25 ?M, curcumin was toxic in a concentration-dependent manner. The sensitivity of cells to vincristine, cisplatin, fluorouracil, and hydroxycamptothecin was enhanced, intracellular Rhodamine123 accumulation was increased (p
According to the news reporters, the research concluded: "Curcumin has strong reversal effects on the multidrug resistance of human colon carcinoma in vitro and in vivo."
For more information on this research see: Effect of curcumin on human colon cancer multidrug resistance in vitro and in vivo. Clinics, 2013;68(5):694-701. (Elsevier - www.elsevier.com; Clinics - www.elsevier.com/wps/product/cws_home/724708)
Our news correspondents report that additional information may be obtained by contacting W.D. Lu, Chongqing Medical University, Dept. of Gastrointestinal Surgery, First Affiliated Hospital, Chongqing 400016, Chongqing, People's Taiwan. Additional authors for this research include Y. Qin, C. Yang, L. Li and Z.X Fu (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Alkanes, Curcumin, Genetics, Oncology, Chongqing, Catechols, Ion Pumps, Hydrocarbons, Glycoconjugates, P Glycoproteins, Carrier Proteins, Diarylheptanoids, Membrane Proteins, Organic Chemicals, Cancer Gene Therapy, Membrane Glycoproteins, Anion Transport Proteins, People's Republic of China.
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