By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Gram-Positive Bacteria is now available. According to news reporting originating in Warsaw, Poland, by NewsRx journalists, research stated, "A chitinolytic actinomycete was isolated from agricultural soil in the centre of Poland. It was identified as Streptomyces rimosus."
The news reporters obtained a quote from the research from the Warsaw University of Life Sciences, "Identification was based on standard biochemical tests and on 16S rRNA gene sequence analysis. The degradation of chitinous substances (shrimp shell waste, crab chitin powder and chitosan) was examined on the basis of oxygen consumption by applying OxiTop (WTW). The enzyme catalyzed the hydrolysis of the disaccharide 4-MU-(GlcNAc)(3) most efficiently and was therefore classified as an endochitinase. The chitinase was purified from culture medium by fractionation with ammonium sulfate and affinity chromatography. The purified proteins were subjected to identification by mass spectrometry. Based on analysis of the resultant fragments of the amino acid sequence of chitinase produced by the examined bacteria, degenerate primers for PCR were designed. The results of our research prove that the substances which are metabolised by S. rimosus the most efficiently are chitosan and the shrimp shell waste. Chitinolytic activity examination showed that shrimp shell waste was the finest inducer of chitinase synthesis. The molecular mass of the purified enzyme was 63 kDa. The highest activity of chitinase was obtained at the temperature of 40 degrees-45 degrees C. Optimal pH for chitinase activity was 7.0. The activity of the purified chitinase was stabilized by Mg2+ ions. The activity of the enzyme was inhibited by Hg2+ and Pb2+ ions. Chitinase inhibited the growth of the fungal phytopathogens Fusarium solani and Alternaria alternata."
According to the news reporters, the research concluded: "The nucleotide sequence of the amplified gene fragment proved to be similar to the sequence of chitinase-encoding genes of the glycoside hydrolases family 18."
For more information on this research see: Biodegradation of chitinous substances and chitinase production by the soil actinomycete Streptomyces rimosus. International Biodeterioration & Biodegradation, 2013;84():104-110. International Biodeterioration & Biodegradation can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; International Biodeterioration & Biodegradation - www.elsevier.com/wps/product/cws_home/405899)
Our news correspondents report that additional information may be obtained by contacting M.S. Brzezinska, Warsaw Univ Life Sci, Dept. of Biochem, Warsaw, Poland. Additional authors for this research include U. Jankiewicz and M. Walczak (see also Gram-Positive Bacteria).
Keywords for this news article include: Warsaw, Poland, Europe, Actinomycetales, Streptomycetaceae, Enzymes and Coenzymes, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Rods
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