By a News Reporter-Staff News Editor at Proteomics Weekly -- Researchers detail new data in Proteomics. According to news reporting originating in Bielefeld, Germany, by NewsRx journalists, research stated, "Microalgae are diverse photosynthetic microbes which offer the potential for production of a number of high value products (HVP) such as pigments, oils, and bio-active compounds. Fast growth rates, ease of photo-autotrophic cultivation, unique metabolic properties and continuing progress in algal transgenics have raised interest in the use of microalgae systems for recombinant protein (RP) production."
The news reporters obtained a quote from the research from the University of Bielefeld, "This work demonstrates the development of an advanced RP production and secretion system for the green unicellular model alga Chlamydomonas reinhardtii. We generated a versatile expression vector that employs the secretion signal of the native extracellular C reinhardtii carbonic anhydrase for efficient RP secretion into the culture medium. Unique restriction sites were placed between the regulatory elements to allow fast and easy sub-cloning of sequences of interest. Positive transformants can rapidly be identified by high-throughput plate-level screens via a coupled Gaussia luciferase marker. The vector was tested in Chlamydomonas wild type CC-1883 (WT) and in the transgene expression transformant UVM4. Compared to the native secretion signal of the Gaussia luciferase, up to 84% higher RP production could be achieved. With this new expression system we could generate transformants that express up to 10 mg RP per liter culture without further optimization. The target RP is found exclusively in culture medium and can therefore easily be isolated and purified."
According to the news reporters, the research concluded: "We conclude that this new expression system will be a valuable tool for many heterologous protein expression applications from C. reinhardtii in the future."
For more information on this research see: Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii. Journal of Biotechnology, 2013;167(2):101-110. Journal of Biotechnology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Biotechnology - www.elsevier.com/wps/product/cws_home/505515)
Our news correspondents report that additional information may be obtained by contacting K.J. Lauersen, University of Bielefeld, Fac Biol, Center Biotechnol CeBiTec, D-33615 Bielefeld, Germany. Additional authors for this research include H. Berger, J.H. Mussgnug and O. Kruse (see also Proteomics).
Keywords for this news article include: Europe, Germany, Bielefeld, Proteomics, Recombinant Proteins
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