By a News Reporter-Staff News Editor at AIDS Vaccine Week -- New research on Immune System Diseases and Conditions is the subject of a report. According to news originating from Durham, North Carolina, by NewsRx correspondents, research stated, "We present an integrated analytical method for analyzing peptide microarray antibody binding data, from normalization through subject-specific positivity calls and data integration and visualization. Current techniques for the normalization of such data sets do not account for nonspecific binding activity."
Our news journalists obtained a quote from the research from Duke University, "A novel normalization technique based on peptide sequence information quickly and effectively reduced systematic biases. We also employed a sliding mean window technique that borrows strength from peptides sharing similar sequences, resulting in reduced signal variability. A smoothed signal aided in the detection of weak antibody binding hotspots. A new principled FDR method of setting positivity thresholds struck a balance between sensitivity and specificity. In addition, we demonstrate the utility and importance of using baseline control measurements when making subject-specific positivity calls."
According to the news editors, the research concluded: "Data sets from two human clinical trials of candidate HIV-1 vaccines were used to validate the effectiveness of our overall computational framework."
For more information on this research see: A computational framework for the analysis of peptide microarray antibody binding data with application to HIV vaccine profiling. Journal of Immunological Methods, 2013;395(1-2):1-13. Journal of Immunological Methods can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Immunological Methods - www.elsevier.com/wps/product/cws_home/506022)
The news correspondents report that additional information may be obtained from G.C. Imholte, Duke University, Medical Center, Duke Human Vaccine Inst, Durham, NC 27710, United States. Additional authors for this research include R. Sauteraud, B. Korber, R.T. Bailer, E.T. Turk, X.Y. Shen, G.D. Tomaras, J.R. Mascola, R.A. Koup, D.C. Montefiori and R. Gottardo (see also Immune System Diseases and Conditions).
Keywords for this news article include: Antibodies, Durham, HIV/AIDS, Peptides, Vaccines, Virology, Immunology, Proteomics, RNA Viruses, Retroviridae, United States, North Carolina, Blood Proteins, HIV Infections, Immunoglobulins, Vertebrate Viruses, Biological Products, Primate Lentiviruses, North and Central America, Clinical Trials and Studies
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