By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Epithelial Cells. According to news reporting originating in Singapore, Singapore, by NewsRx journalists, research stated, "Considerable interest has been generated for the development of suitable corneal endothelial graft alternatives through cell-tissue engineering, which can potentially alleviate the shortage of corneal transplant material. The advent of less invasive suture-less key-hole surgery options such as Descemet's Stripping Endothelial Keratoplasty (DSEK) and Descemet's Membrane Endothelial Keratoplasty (DMEK), which involve transplantation of solely the endothelial layer instead of full thickness cornea, provide further impetus for the development of alternative endothelial grafts for clinical applications."
The news reporters obtained a quote from the research from the Institute of Medical Biology, "A major challenge for this endeavor is the lack of specific markers for this cell type. To identify genes that reliably mark corneal endothelial cells (CECs) in vivo and in vitro, we performed RNA-sequencing on freshly isolated human CECs (from both young and old donors), CEC cultures, and corneal stroma. Gene expression of these corneal cell types was also compared to that of other human tissue types. Based on high throughput comparative gene expression analysis, we identified a panel of markers that are: i) highly expressed in CECs from both young donors and old donors; ii) expressed in CECs in vivo and in vitro; and iii) not expressed in corneal stroma keratocytes and the activated corneal stroma fibroblasts. These were SLC4A11, COL8A2 and CYYR1."
According to the news reporters, the research concluded: "The use of this panel of genes in combination reliably ascertains the identity of the CEC cell type."
For more information on this research see: High throughput gene expression analysis identifies reliable expression markers of human corneal endothelial cells. Plos One, 2013;8(7):e67546. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news correspondents report that additional information may be obtained by contacting Z. Chng, ASTAR Institute of Medical Biology, Singapore, Singapore. Additional authors for this research include G.S. Peh, W.B. Herath, T.Y. Cheng, H.P. Ang, K.P. Toh, P. Robson, J.S. Mehta and A. Colman (see also Epithelial Cells).
Keywords for this news article include: Asia, Epithelial Cells, Machine Learning, Endothelial Cells, Emerging Technologies, Gene Expression Analysis.
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