By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on DNA Research is now available. According to news reporting originating in Irvine, California, by NewsRx journalists, research stated, "Polydopamine (PDA) films were fabricated on thin film gold substrates in a single-step polymerization deposition process from dopamine solutions and then employed in the construction of robust DNA microarrays for the ultrasensitive detection of biomolecules with nanoparticle-enhanced surface plasmon resonance (SPR) imaging. PDA multilayers with thicknesses varying from 1 to 5 nm were characterized with a combination of scanning angle SPR and AFM experiments, and 1.3 +/- 0.2 nm PDA multilayers were chosen as an optimal thickness for the SPR imaging measurements."
The news reporters obtained a quote from the research from the University of California, "DNA microarrays were then fabricated by the reaction of amine-functionalized single-stranded DNA (ssDNA) oligonucleotides with PDA-modified gold thin film microarray elements, and were subsequently employed in SPR imaging measurements of DNA hybridization adsorption and protein DNA binding. Concurrent control experiments with non-complementary ssDNA sequences demonstrated that the adhesive PDA multilayer was also able to provide good resistance to the nonspecific binding of biomolecules."
According to the news reporters, the research concluded: "Finally, a series of SPR imaging measurements of the hybridization adsorption of DNA-modified gold nanoparticles onto mixed sequence DNA microarrays were used to confirm that the use of PDA multilayer films is a simple, rapid, and versatile method for fabricating DNA microarrays for ultrasensitive nanoparticle-enhanced SPR imaging biosensing."
For more information on this research see: Fabrication of DNA Microarrays on Polydopamine-Modified Gold Thin Films for SPR Imaging Measurements. Langmuir, 2013;29(34):10868-10873. Langmuir can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Langmuir - www.pubs.acs.org/journal/langd5)
Our news correspondents report that additional information may be obtained by contacting J.B. Wood, University of California, Dept. of Chem, Irvine, CA 92697, United States. Additional authors for this research include M.W. Szyndler, A.R. Halpern, K. Cho and R.M. Corn (see also DNA Research).
Keywords for this news article include: Irvine, California, DNA Research, United States, North and Central America, Surface Plasmon Resonance
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