By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in DNA Research. According to news reporting out of Jinan, People's Republic of China, by NewsRx editors, research stated, "A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase (hpt) marker gene."
Our news journalists obtained a quote from the research from the Shandong Academy of Agriculture Science, "Using this binary vector, we constructed two vectors that expressed inverted-repeat (ER) structures targeting the rice stripe virus (RSV) coat protein (CP) gene and the special-disease protein (SP) gene. Transgenic rice lines were obtained via Agrobacterium-mediated transformation. Seven independent clones harbouring both the hpt marker gene and the target genes (RSV CP or SP) were obtained in the primary transformants of pDTRSVCP and pDTRSVSP, respectively. The segregation frequencies of the target gene and the marker gene in the T-1 plants were 8.72% for pDTRSVCP and 12.33% for pDTRSVSP. Two of the pDTRSVCP lines and three pDTRSVSP lines harbouring the homozygous target gene, but not the hpt gene, were strongly resistant to RSV. A molecular analysis of the resistant transgenic plants confirmed the stable integration and expression of the target genes."
According to the news editors, the research concluded: "The resistant transgenic plants displayed lower levels of the transgene transcripts and specific small interfering RNAs, suggesting that RNAi induced the viral resistance."
For more information on this research see: Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Journal of Biosciences, 2013;38(3):573-581. Journal of Biosciences can be contacted at: Indian Acad Sciences, C V Raman Avenue, Sadashivanagar, P B #8005, Bangalore 560 080, India. (Springer - www.springer.com; Journal of Biosciences - www.springerlink.com/content/0250-5991/)
Our news journalists report that additional information may be obtained by contacting Y.Y. Jiang, Shandong Academy Agr Sci, Rice Sci Res Inst, Jinan 250100, People's Republic of China. Additional authors for this research include L. Sun, M.S. Jiang, K.D. Li, Y.Z. Song and C.X. Zhu (see also DNA Research).
Keywords for this news article include: Asia, Jinan, Viral DNA, DNA Research, People's Republic of China
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