By a News Reporter-Staff News Editor at Cancer Weekly -- Investigators discuss new findings in Nanotechnology. According to news reporting originating in Ann Arbor, Michigan, by NewsRx journalists, research stated, "Epithelial ovarian cancer cells enhance their ability to migrate and invade through the epithelial-mesenchymal transition (EMT), resulting in cell seeding and metastasis in the peritoneal cavity and onto adjacent organ surfaces. It has been speculated that cytoskeletal dynamics, such as those of the actin filament, play a role in enhanced cell motility; however, direct evidence has not been provided."
The news reporters obtained a quote from the research from the University of Michigan, "Herein, we have directly measured pico- to nanonewton-scale mechanical forces generated by actin dynamics of ovarian cancer SKOV-3 cells upon binding of integrin alpha 5 beta 1 to fibronectin (FN), i.e., formation of a focal adhesion, using real-time atomic force microscopy (AFM) in a force spectroscopy mode. The dendrimer surface chemistry through which FN was immobilized on the AFM probe surfaces further enhanced the sensitivity of the force measurement by 1.5-fold. Post-EMT SKOV-3 cells, induced by transforming growth factor-beta, generated larger focal adhesion mechanical forces (17 and 41 nN before and after EMT, respectively) with migration faster than that of pre-EMT cells. Importantly, 22% of the forces transmitted through a single FN-integrin alpha 5 beta 1 pair from post-EMT cells were shown to be sufficient to rupture the binding between FN and integrin alpha 5 beta 1 on the cells, a result which is not observed on pre-EMT cells. This implies that post-EMT cells, by generating forces strong enough to break the FN-integrin binding, migrate and metastasize beyond the ovary, whereas pre-EMT cancer cells are confined in the ovary without such force generation."
According to the news reporters, the research concluded: "These results demonstrate quantitative and direct evidence for the role of actin dynamics in the enhanced motility of post-EMT ovarian cancer cells, providing a fundamental insight into the mechanism of ovarian cancer metastasis."
For more information on this research see: Epithelial-Mesenchymal Transition Enhances Nanoscale Actin Filament Dynamics of Ovarian Cancer Cells. Journal of Physical Chemistry B, 2013;117(31):9233-9240. Journal of Physical Chemistry B can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Physical Chemistry B - www.pubs.acs.org/journal/jpcbfk)
Our news correspondents report that additional information may be obtained by contacting S. Lee, University of Michigan, Dept. of Chem, Ann Arbor, MI 48109, United States. Additional authors for this research include Y. Yang, D. Fishman, M.M.B. Holl and S. Hone (see also technology.html">Nanotechnology).
Keywords for this news article include: Cancer, Michigan, Oncology, Ann Arbor, Nanoscale, United States, Nanotechnology, Emerging Technologies, North and Central America
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