By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting from Changsha, People's Republic of China, by NewsRx journalists, research stated, "A novel, high sensitive, and specific DNA assay based on gold nanoparticle (AuNP) colorimetric detection and hybridization chain reaction (HCR) amplification has been demonstrated in this article. Two hairpin auxiliary probes were designed with single-stranded DNA (ssDNA) sticky ends which stabilize AuNPs and effectively prevent them from salt-induced aggregation."
The news correspondents obtained a quote from the research from Hunan University, "The target DNA hybridized with the hairpin auxiliary probes and triggered the formation of extended double-stranded DNA (dsDNA) polymers through HCR. As a result, the formed dsDNA polymers provide less stabilization without ssDNA sticky ends, and AuNPs undergo aggregation when salt concentration is increased. Subsequently, a pale purple-to-blue color variation is observed in the colloid solution. The system is simple in design and convenient in operation. The novel strategy eliminates the need for enzymatic reactions, separation processes, chemical modifications, and sophisticated instrumentation. The detection and discrimination process can be seen with the naked eye. The detection limit of this method is lower than or at least comparable to previous AuNP-based methods."
According to the news reporters, the research concluded: "Importantly, the protocol offers high selectivity for the determination between perfectly matched target oligonucleotides and targets with single base-pair mismatches."
For more information on this research see: Enzyme-Free Colorimetric Detection of DNA by Using Gold Nanoparticles and Hybridization Chain Reaction Amplification. Analytical Chemistry, 2013;85(16):7689-7695. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting P. Liu, Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Coll Chem & Chem Engn, Key Lab Bionanotechnol & Mol Engn Hunan Prov, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include X.H. Yang, S. Sun, Q. Wang, K.M. Wang, J. Huang, J.B. Liu and L.L. He (see also DNA Research).
Keywords for this news article include: Asia, Changsha, DNA Research, Nanotechnology, Gold Nanoparticles, Emerging Technologies, People's Republic of China
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