By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Epithelial Cells are discussed in a new report. According to news reporting out of Milan, Italy, by NewsRx editors, research stated, "Metal oxide NPs are abundantly produced in nanotech industries and are emitted in several combustion processes, suggesting the need to characterize their toxic impact on the human respiratory system. The acute toxicity and the morphological changes induced by copper oxide and titanium dioxide NPs (nCuO and nTiO(2)) on the human alveolar cell line A549 are here investigated."
Our news journalists obtained a quote from the research from the University of Milan, "Cell viability and oxidative stress have been studied in parallel with NP internalization and cell ultrastructural modifications. TiO2 NPs were abundantly internalized by cells through the endocytic pathway, even they did not induce cell death and ultrastructural lesions. Only after 24 h cells were affected by an abundant NP internalization presenting a consequent altered morphology. High cytotoxicity, oxidative stress and severe ultrastructural damages were produced by nCuO, since cell membrane and mitochondria resulted to be heavily affected, even at early exposure time. nCuO-induced toxicity has been interpreted as a consequence of both NPs reactivity and copper ions dissolution in lysosomal compartments, even the free NPs, scattered throughout all the cell compartments, might contribute to the toxicity. The antioxidant N-acetylcysteine was effective in recovering nCuO exposed cells viability and Bafilomycin A1 inhibited copper ions release in phagolysosomes and significantly rescued cells, suggesting a relevant cytotoxic mechanism relative to oxidative damages and authophagic cell death, together with NP internalization and dissolution. Our results support the previous data reporting CuO NPs are highly cytotoxic and genotoxic, and associate their toxic effects with their cell penetration and interaction with various compartments."
According to the news editors, the research concluded: "The so-called 'Trojan horse' mechanism and autophagy, are involved in nCuO-induced cell death, even a further research is needed to explain the events occurring at early exposure time."
For more information on this research see: The modality of cell-particle interactions drives the toxicity of nanosized CuO and TiO2 in human alveolar epithelial cells. Toxicology Letters, 2013;222(2):102-116. Toxicology Letters can be contacted at: Elsevier Ireland Ltd, Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland. (Elsevier - www.elsevier.com; Toxicology Letters - www.elsevier.com/wps/product/cws_home/505519)
Our news journalists report that additional information may be obtained by contacting E. Moschini, University of Milan, CIMA, Interdept Adv Microscopy Center, I-20133 Milan, Italy. Additional authors for this research include M. Gualtieri, M. Colombo, U. Fascio, M. Camatini and P. Mantecca (see also Epithelial Cells).
Keywords for this news article include: Milan, Italy, Europe, Epithelial Cells
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC