By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Surface-Active Agents. According to news reporting originating in Storrs, Connecticut, by NewsRx journalists, research stated, "The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins."
The news reporters obtained a quote from the research from the University of Connecticut, "Exposure to detergents, however, can destabilize many membrane proteins and result in a loss of function. Amphipathic copolymers have recently been used to stabilize membrane proteins and complexes following suitable detergent extraction. However, the ability of these copolymers to extract proteins directly from native lipid bilayers for subsequent reconstitution and characterization has not been explored. The styrene-maleic acid (SMA) copolymer effectively solubilized membranes of isolated mitochondria and extracted protein complexes. Membrane complexes were reconstituted into polymer-bound nanoscale discs along with endogenous lipids. Using respiratory Complex IV as a model, these particles were shown to maintain the enzymatic activity of multicomponent electron transporting complexes. We report a novel process for reconstituting fully operational protein complexes directly from cellular membranes into nanoscale lipid bilayers using the SMA copolymer."
According to the news reporters, the research concluded: "This facile, single-step strategy obviates the requirement for detergents and yields membrane complexes suitable for structural and functional studies."
For more information on this research see: A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs. Bmc Biotechnology, 2013;13():41. (BioMed Central - www.biomedcentral.com/; Bmc Biotechnology - www.biomedcentral.com/bmcbiotechnol/)
Our news correspondents report that additional information may be obtained by contacting A.R. Long, Dept. of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Rd, Storrs, Connecticut 06269, United States. Additional authors for this research include C.C. O'Brien, K. Malhotra, C.T. Schwall, A.D. Albert, A. Watts and N.N Alder (see also Surface-Active Agents).
Keywords for this news article include: Storrs, Peptides, Nanoscale, Detergents, Connecticut, Amino Acids, United States, Nanotechnology, Membrane Proteins, Emerging Technologies, Surface Active Agents, Surface-Active Agents, North and Central America.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC