By a News Reporter-Staff News Editor at Stem Cell Week -- Current study results on Stem Cell Research have been published. According to news reporting out of Beijing, People's Republic of China, by NewsRx editors, research stated, "Whether differentiation of induced pluripotent stem cells (iPSCs) in ischemic myocardium enhances their immunogenicity, thereby increasing their chance for rejection, is unclear. Here, we dynamically demonstrated the immunogenicity and rejection of iPSCs in ischemic myocardium using bioluminescent imaging (BLI)."
Our news journalists obtained a quote from the research from the Institute of Basic Medical Sciences, "Murine iPSCs were transduced with a tri-fusion (TF) reporter gene consisting of firefly luciferase-red fluorescent protein-truncated thymidine kinase (fluc-mrfp-tTK). Ascorbic acid (Vc) were used to induce iPSCs to differentiate into cardiomyocytes (CM). iPSCs and iPS-CMs were intramyocardially injected into immunocompetent or immunosuppressed allogenic murine with myocardial infarction. BLI was performed to track transplanted cells. Immune cell infiltration was evaluated by immunohistochemistry. Syngeneic iPSCs were also injected and evaluated. The results demonstrated that undifferentiated iPSCs survived and proliferated in allogenic immunocompetent recipients early post-transplantation, accompanying with mild immune cell infiltration. With in vivo differentiation, a progressive immune cell infiltration could be detected. While transplantation of allogenic iPSC-CMs were observed an acute rejection from receipts. In immune-suppressed recipients, the proliferation of iPSCs could be maintained and intramyocardial teratomas were formed. Transplantation of syngeneic iPSCs and iPSC-CMs were also observed progressive immune cell infiltration."
According to the news editors, the research concluded: "This study demonstrated that iPSC immunogenicity increases with in vivo differentiation, which will increase their chance for rejection in iPSC-based therapy."
For more information on this research see: Molecular imaging of induced pluripotent stem cell immunogenicity with in vivo development in ischemic myocardium. Plos One, 2013;8(6):e66369. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting Z. Liu, Dept. of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Taiwan. Additional authors for this research include X. Wen, H. Wang, J. Zhou, M. Zhao, Q. Lin, Y. Wang, J. Li, D. Li, Z. Du, A. Yao, F. Cao and C. Wang (see also Stem Cell Research).
Keywords for this news article include: Asia, Heart, Beijing, Myocardium, Nanotechnology, Molecular Imaging, Stem Cell Research, Emerging Technologies, People's Republic of China.
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