By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Proteins are presented in a new report. According to news reporting originating in Morgantown, West Virginia, by NewsRx journalists, research stated, "Electron transfer in cytochrome P450 enzymes is a fundamental process for activity. It is difficult to measure electron transfer in these enzymes because under the conditions typically used they exist in a variety of states."
The news reporters obtained a quote from the research from West Virginia University, "Using nanotechnology-based techniques, gold conducting nanopillars were constructed in an indexed array. The P450 enzyme CYP2C9 was attached to each of these nanopillars, and conductivity measurements made using conducting probe atomic force microscopy under constant force conditions. The conductivity measurements were made on CYP2C9 alone and with bound substrates, a bound substrate-effector pair, and a bound inhibitor. Fitting of the data with the Poole-Frenkel model indicates a correlation between the barrier height for electron transfer and the ease of CYP2C9-mediated metabolism of the bound substrates, though the spin state of iron is not well correlated."
According to the news reporters, the research concluded: "The approach described here should have broad application to the measurement of electron transfer in P450 enzymes and other metalloenzymes."
For more information on this research see: Measurement of electron transfer through cytochrome P450 protein on nanopillars and the effect of bound substrates. Journal of the American Chemical Society, 2013;135(10):3834-40. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)
Our news correspondents report that additional information may be obtained by contacting J.E. Jett, Basic Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506-9530, United States. Additional authors for this research include D. Lederman, L.A. Wollenberg, D. Li, D.R. Flora, C.D. Bostick, T.S. Tracy and P.M Gannett (see also Proteins).
Keywords for this news article include: Morgantown, Cytochromes, Hemeproteins, West Virginia, United States, Enzymes and Coenzymes, North and Central America.
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