By a News Reporter-Staff News Editor at Proteomics Weekly -- Investigators publish new report on Proteomics. According to news originating from Boston, Massachusetts, by NewsRx correspondents, research stated, "Often peptides used in synthesis of radiopharmaceutical PET tracers are lipophilic and adhere to the walls of container closure systems (CCS) such that costly peptide and product are not fully recoverable after synthesis occurs. This investigation compares a standard United States Pharmacopeia (USP) Type I borosilicate glass CCS to a cyclic polyolefin copolymer Crystal Zenith (CZ) CCS, for Ga-68-chloride and Ga-68-DOTATOC ([Ga-68] Ga-DOTA-D-Phe1-Tyr3-octreotide) retention in the reaction vial after labeling."
Our news journalists obtained a quote from the research from Massachusetts General Hospital, "(68)Gallium labeling of DOTATOC was conducted by adding Ga-68-chloride, 2 M HEPES (4-(2-hydroxyethyl)piperazine-l-ethanesulfonic acid) or 0.75 M sodium acetate, and 1-30 mu g of DOTATOC into the CZ or glass CCS. The reaction mixture was heated for 15 min and cooled to room temperature. The crude reaction mixture was then withdrawn via syringe, for final processing. The CCS was then assayed using a dose calibrator to determine the amount of retained Ga-68-DOTATOC. Statistical significance was assessed using an unpaired Students t-test. In all experiments (n=72) with various amounts of peptide and different buffering systems, the CZ CCS retained less activity than the glass CCS. Using 2 M HEPES and 15 mu g or 30 mu g of DOTATOC, the CZ CCS retained approximately 10% less of the labeled DOTATOC compared to the glass CCS (p < 0.05). Utilizing either a sodium acetate or a HEPES buffering system with 15 mu g or 30 mu g of DOTATOC, the CZ CCS retained approximately 2.5% less of the total reaction activity compared to the glass CCS (p < 0.05). Product yield was equivalent in glass and CZ CCS under the same reaction conditions. Both the CZ and glass vials showed no retention of Ga-68-chloride."
According to the news editors, the research concluded: "For applications involving the labeling of peptides such as Ga-68-DOTATOC, the CZ CCS compared to the glass CCS, results in an improved recovery of product."
For more information on this research see: A container closure system that allows for greater recovery of radiolabeled peptide compared to the standard borosilicate glass system. Applied Radiation and Isotopes, 2013;80():99-102. Applied Radiation and Isotopes can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Applied Radiation and Isotopes - www.elsevier.com/wps/product/cws_home/228)
The news correspondents report that additional information may be obtained from A.K. Leece, Massachusetts General Hospital, Div Nucl Med & Mol Imaging, Dept. of Radiol, Boston, MA 02114, United States. Additional authors for this research include P. Heidari, D.L. Yokell and U. Mahmood (see also Proteomics).
Keywords for this news article include: Boston, Anions, Peptides, Proteins, Chlorides, Proteomics, Massachusetts, United States, Hydrochloric Acid, North and Central America
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