By a News Reporter-Staff News Editor at Drug Week -- New research on Nanoparticles is the subject of a report. According to news reporting out of Karnataka, India, by NewsRx editors, research stated, "Potential use of siRNA as therapeutic agent has elicited a great deal of interest. However, insufficient cellular uptake and poor stability limited its application in therapeutics."
Our news journalists obtained a quote from the research from Manipal University, "In our earlier study, we prepared PLGA nanoparticles for effective delivery of siRNA targeting Bcl-2 gene to block its expression. Purpose of the present study was to improve effectiveness of PLGA nanoformulation of siRNA targeting anti-apoptotic Bcl-2 gene through chitosan coating. We prepared chitosan coated PLGA nanoparticles by using the double emulsion solvent diffusion (DESE) method. Characterization of prepared chitosan coated nanoformulation was done followed by cytotoxicity studies, expression studies and in vivo studies. Particle size of chitosan coated nanoparticles was found to be increased compared to PLGA nanoparticles from 244 to 319 nm. Surface charge of chitosan coated nanoparticles was found to be positive facilitating transfection of nanoformulation into cells. In vitro studies indicated increased transfection of nanoparticles resulting in effective silencing of Bcl-2. Marked apoptotic lesions were observed in nuclear staining studies. On comparison of the results from the present study with those of previous study, it was found that the extent of silencing of Bcl-2 gene by PLGA nanoformulation has improved significantly through chitosan coating."
According to the news editors, the research concluded: "In vivo studies showed significant tumor regression in animals treated with chitosan coated PLGA nanoformulation of siRNA."
For more information on this research see: Improvement of therapeutic efficacy of PLGA nanoformulation of siRNA targeting anti-apoptotic Bcl-2 through chitosan coating. European Journal of Pharmaceutical Sciences, 2013;48(4-5):611-8. (Elsevier - www.elsevier.com; European Journal of Pharmaceutical Sciences - www.elsevier.com/wps/product/cws_home/523997)
Our news journalists report that additional information may be obtained by contacting H.V. Jagani, Dept. of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, India. Additional authors for this research include V.R. Josyula, V.R. Palanimuthu, R.C. Hariharapura and S.S Gang (see also Nanoparticles).
Keywords for this news article include: Asia, India, Karnataka, Nanotechnology, Emerging Technologies.
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