By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Cellular Structures is now available. According to news originating from New York City, New York, by NewsRx correspondents, research stated, "Platforms which can display cell membrane ligands and receptors as a microarray library of probes for screening against a target are essential tools in drug discovery, biomarker identification, and pathogen detection. Membrane receptors and ligands require their native bilayer environment to retain their selectivity and binding affinity, and this complicates displaying them in a microarray platform."
Our news journalists obtained a quote from the research from the City University of New York, "In this study, a design is developed in which the probes are first incorporated in supported lipid bilayers formed around micron-sized particles (lipobeads), and the microbeads themselves are then arrayed on a surface by hydrodynamic capture in a microfluidic obstacle course of traps. The traps are 'V' shaped open enclosures, which are arranged in a wide channel of a microfluidic device, and capture the lipobeads (slightly smaller than the channel height) as they are streamed through the course. Screening assays are undertaken directly in the device after assembly, by streaming a fluorescently labeled target through the device and detecting the bead fluorescence. Conditions are first established for which the supported bilayers on the bead surface remain intact during the capture and assay steps, using fluorescent tags in the bilayer to infer bilayer integrity. Numerical calculations of the hydrodynamic drag coefficient on the entrapped beads are presented in conjunction with the stability experiments to develop criteria for the bilayer stability as a function of the screening assay perfusion rate. Simulations of the flow streamlines are also presented to quantify the trapping efficiency of the obstacle course. Screening assays are illustrated, assaying fluorescently labeled NeutrAvidin with biotin, and labeled cholera toxin with its ganglioside binding ligand, GM1. Sequential capturing of sets of lipobeads (one at a time, and with each set bearing a different probe), followed by indexing the bead positions after each set is entrapped, allows for the construction of an indexed array of multiple probes without the need for particle encoding and is illustrated using the NeutrAvidin-biotin pair."
According to the news editors, the research concluded: "Finally, the lipobead platform is used for quantitatively measuring the kinetic rate constants for the binding of a probe (biotin) to a target (NeutrAvidin)."
For more information on this research see: A lipobead microarray assembled by particle entrapment in a microfluidic obstacle course and used for the display of cell membrane receptors. Lab On a Chip - Miniaturisation for Chemistry and Biology, 2013;13(15):3041-60 (see also Cellular Structures).
The news correspondents report that additional information may be obtained from X. Chen, Levich Institute and Dept. of Chemical Engineering, The City College of the City University of New York, New York, New York 10031, United States. Additional authors for this research include S. Shojaei-Zadeh, M.L. Gilchrist and C. Maldarelli.
Keywords for this news article include: New York City, United States, Cell Membrane, Cellular Structures, North and Central America.
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