By a News Reporter-Staff News Editor at Biotech Business Week -- Investigators publish new report on Autoimmune Diseases and Disorders. According to news reporting out of Gothenburg, Sweden, by NewsRx editors, research stated, "MicroRNA are small noncoding RNA molecules that are involved in the control of gene expression. To investigate the role of microRNA in multiple sclerosis (MS), we performed genome-wide expression analyses of mRNA and microRNA in T-cells from MS patients and controls."
Our news journalists obtained a quote from the research from the University of Gothenburg, "Heparin-anticoagulated peripheral blood was collected from MS-patients and healthy controls followed by isolation of T-cells. MicroRNA and RNA from T-cells was prepared and hybridized to Affymetrix miR 2.0 array and Affymetrix U133Plus 2.0 Human Genome array (Santa Clara, CA), respectively. Verifications were performed with real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). We identified 2,452 differentially expressed genes and 21 differentially expressed microRNA between MS patients and controls. By Kolmogorov-Smirnov test, 20 of 21 differentially expressed microRNA were shown to affect the expression of their target genes, many of which were involved in the immune system. Tumor necrosis factor ligand superfamily member 14 (TNFSF14) was a microRNA target gene significantly decreased in MS. The differential expression of mir-494, mir-197 and the predicted microRNA target gene TNFSF14 was verified by real-time PCR and ELISA."
According to the news editors, the research concluded: "These findings indicate that microRNA may be important regulatory molecules in T-cells in MS."
For more information on this research see: MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS). Bmc Immunology, 2013;14():32. (BioMed Central - www.biomedcentral.com/; Bmc Immunology - www.biomedcentral.com/bmcimmunol/)
Our news journalists report that additional information may be obtained by contacting M. Jernas, Dept. of Molecular and Clinical Medicine, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden. Additional authors for this research include C. Malmestrom, M. Axelsson, I. Nookaew, H. Wadenvik, J. Lycke and B. Olsson (see also Autoimmune Diseases and Disorders).
Keywords for this news article include: Sweden, Europe, Genetics, Neurology, Gothenburg, Neuroimmunology, Multiple Sclerosis, Demyelinating Diseases, Biotechnology Companies, Autoimmune Diseases and Disorders, CNS Demyelinating Autoimmune Diseases, Autoimmune Diseases of the Nervous System.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2013, NewsRx LLC