By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Microchemical Research. According to news originating from Cordoba, Spain, by NewsRx correspondents, research stated, "We report on a method for the determination of soy proteins in food samples via dispersive solid-phase immunoextraction using gold-coated magnetic nanoparticles (NPs) as a support. Soy proteins were first extracted using anti-soy protein antibodies immobilized on the NPs, and then quantified by measuring the increase in fluorescence of the long-wavelength fluorophore cresyl violet in the presence of the anionic surfactant sodium dodecyl sulfate at neutral pH in a flow system."
Our news journalists obtained a quote from the research from the University of Cordoba, "The method involves the use of two standard or sample aliquots. The fluorescence intensity of one aliquot is directly measured whereas that of the other aliquot is measured after immunoextraction. The difference between the peak heights of both aliquots serves as the analytical information that is directly proportional to the protein concentration. The limit of detection is 0.35 mg L-1, the linear range is from 1 to 15 mg L-1, and the relative standard deviation is < 5 %. Proteins such as bovine serum albumin and globulins do not interfere at the same concentration level."
According to the news editors, the research concluded: "The method was applied to the analysis of soy-based beverages and gave recoveries in the range between 80.0 and 107.3 %."
For more information on this research see: Determination of soy proteins in food samples by dispersive solid-phase immunoextraction and dynamic long-wavelength fluorometry. Microchimica Acta, 2013;180(13-14):1279-1286. Microchimica Acta can be contacted at: Springer Wien, Sachsenplatz 4-6, PO Box 89, A-1201 Wien, Austria. (Springer - www.springer.com; Microchimica Acta - www.springerlink.com/content/0026-3672/)
The news correspondents report that additional information may be obtained from M.A. Molina-Delgado, University of Cordoba, Dept. of Analyt Chem, Res Inst Fine Chem & Nanochem IUQFN, E-14071 Cordoba, Spain. Additional authors for this research include M.P. Aguilar-Caballos and A. Gomez-Hens (see also Microchemical Research).
Keywords for this news article include: Spain, Europe, Cordoba, Peptides, Proteins, Amino Acids, Microchemical Research
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