By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Hydrochloric Acid have been published. According to news reporting originating in Leiden, Netherlands, by NewsRx journalists, research stated, "A microfluidic chip is presented for lysis and one-step RNA purification from bacteria. Bacteria are lysed by joule-heating followed by a gel electrophoresis step for clean-up and subsequent elution of small RNA."
The news reporters obtained a quote from the research from Leiden University, "Bubble formation during electrophoresis at constant current is suppressed through the use of a silver chloride cathode and a silver anode. To prevent silver chloride sediment in the bulk solution, the anode was immersed in a saturated chloride solution. Salt bridges in the form of polyacrylamide gels are used that could be precisely patterned with the help of phaseguides. Bubble-free actuation could be performed for more than 20 min under a constant current. For longer actuation times, cathodic silver-chloride became depleted and a silver-chloride sediment formed in the anodic microchamber at increasing distance from the anode with time. The chip functioning was verified by extraction of transfer-messenger RNA from Escherichia coli and subsequent amplification using reverse transcription real-time PCR. Incorporation of salt bridges enables effective bubble free actuation of Ag/AgCl electrodes in a microfluidic chip."
According to the news reporters, the research concluded: "This opens up new possibilities in a surge towards fully integrated diagnostic cartridges that are miniaturized and disposable."
For more information on this research see: Bubble-free electrode actuation for micro-preparative scale electrophoresis of RNA. Lab On a Chip - Miniaturisation for Chemistry and Biology, 2013;13(15):2931-6 (see also Hydrochloric Acid).
Our news correspondents report that additional information may be obtained by contacting P. Vulto, Leiden Academic Centre for Drug Research (LACDR), Division of Analytical Biosciences, Leiden University, Einsteinweg 55, 2333CC, Leiden, Netherlands. Additional authors for this research include P. Kuhn and G.A Urban.
Keywords for this news article include: Leiden, Europe, Anions, Chemicals, Chemistry, Netherlands, Silver Chloride, Hydrochloric Acid.
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