By a News Reporter-Staff News Editor at Stem Cell Week -- Current study results on Stem Cell Research have been published. According to news reporting originating in London, United Kingdom, by NewsRx journalists, research stated, "Human umbilical cord blood (hUCB) has been proposed to contain not only haematopoietic stem cells, but also a rare pluripotent embryonic-like stem cell (ELSc) population that is negative for hematopoietic markers (Lin(-)CD45(-)) and expresses markers typical of pluripotent cells. The aim of this work was to isolate, characterise and expand this ELSc fraction from hUCB, as it may provide a valuable cell source for regenerative medicine applications."
The news reporters obtained a quote from the research from Anthony Nolan Research Institute, "We found that we could indeed isolate a Lin(-)CD45(-) population of small cells (3-10 m diameter) with a high nucleus to cytoplasm ratio that expressed the stem cell markers CD34 and CXCR4. However, in contrast to some previous reports, this fraction was not positive for CD133. Furthermore, although these cells expressed transcripts typical of pluripotent cells, such as SOX2, OCT3/4, and NANOG, they were not able to proliferate in any of the culture media known to support stem cell growth that we tested. Further analysis of the Lin(-)CD45(-) population by flow cytometry showed the presence of a Lin(-)CD45(-)Nestin(+) population that were also positive for CD34 (20%) but negative for CXCR4. These data suggest that the Lin(-)CD45(-) stem cell fraction present in the cord blood represents a small heterogeneous population with phenotypic characteristics of stem cells, including a Lin(-)CD45(-)Nestin(+) population not previously described. This study also suggests that heterogeneity within the Lin(-)CD45(-) cell fraction is the likely explanation for differences in the hUCB cell populations described by different groups that were isolated using different methods. These populations have been widely called 'embryonic-like stem cell' on the basis of their phenotypical similarity to embryonic stem cells."
According to the news reporters, the research concluded: "However, the fact they do not seem to be able to self-renew casts some doubt on their identity, and warns against defining them as 'embryonic-like stem cell' at this stage."
For more information on this research see: Cord blood Lin(-)CD45(-) embryonic-like stem cells are a heterogeneous population that lack self-renewal capacity. Plos One, 2013;8(6):e67968. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news correspondents report that additional information may be obtained by contacting C. Alvarez-Gonzalez, Anthony Nolan Research Institute, London, UK. Additional authors for this research include R. Duggleby, B. Vagaska, S. Querol, S.G. Gomez, P. Ferretti and A. Madrigal (see also Stem Cell Research).
Keywords for this news article include: Biomedical Engineering, Biomedicine, London, Europe, Cord Blood, United Kingdom, Bioengineering, Blood Transfusion, Stem Cell Research, Transfusion Medicine, Regenerative Medicine.
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