By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Life Science Research. According to news reporting originating from Hubei, People's Republic of China, by NewsRx correspondents, research stated, "Herein, we describe the generation of a monomeric large Stokes shift (LSS) red fluorescent protein, mBeRFP, with excitation and emission peaks at 446 and 615 nm, respectively. Compared with two previously reported LSS-RFPs (mKeima and LSS-mKate2), mBeRFP is approximately three times brighter."
Our news editors obtained a quote from the research from the Huazhong University of Science and Technology, "In addition, mBeRFP is characterized by improved photostability, rapid maturation, an extended lifetime, and a monomeric nature. Additionally, mBeRFP can be paired with the Alexa 647 dye as a FRET donor to detect caspase 3 activity. This FRET pair has an extremely dynamic range and a large Forster radius (approximately 6.5 nm). To demonstrate the applicability of mBeRFP for imaging in living cells, we performed dual-color imaging of mBeRFP and CFP simultaneously excited by a single excitation source, and we demonstrated that these fluorescent proteins allow the clear visualization of the dynamics of Bax during cancer cell apoptosis."
According to the news editors, the research concluded: "Thus, mBeRFP appears to be particularly useful for cellular imaging applications."
For more information on this research see: mBeRFP, an improved large stokes shift red fluorescent protein. Plos One, 2013;8(6):e64849. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news editors report that additional information may be obtained by contacting J. Yang, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, People's Taiwan. Additional authors for this research include L. Wang, F. Yang, H. Luo, L. Xu, J. Lu, S. Zeng and Z. Zhang (see also Life Science Research).
Keywords for this news article include: Asia, Hubei, Life Science Research, People's Republic of China.
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