By a News Reporter-Staff News Editor at Information Technology Newsweekly -- Investigators publish new report on Applied Bioinformatics. According to news reporting originating from Milano, Italy, by VerticalNews correspondents, research stated, "Next-Generation Sequencing (NGS) technology has exceptionally increased the ability to sequence DNA in a massively parallel and cost-effective manner. Nevertheless, NGS data analysis requires bioinformatics skills and computational resources well beyond the possibilities of many 'wet biology' laboratories."
Our news editors obtained a quote from the research from the University of Milan, "Moreover, most of projects only require few sequencing cycles and standard tools or workflows to carry out suitable analyses for the identification and annotation of genes, transcripts and splice variants found in the biological samples under investigation. These projects can take benefits from the availability of easy to use systems to automatically analyse sequences and to mine data without the preventive need of strong bioinformatics background and hardware infrastructure. To address this issue we developed an automatic system targeted to the analysis of NGS data obtained from large-scale transcriptome studies. This system, we named NGS-Trex (NGS Transcriptome profile explorer) is available through a simple web interface http://www.ngs-trex.org and allows the user to upload raw sequences and easily obtain an accurate characterization of the transcriptome profile after the setting of few parameters required to tune the analysis procedure. The system is also able to assess differential expression at both gene and transcript level (i.e. splicing isoforms) by comparing the expression profile of different samples.By using simple query forms the user can obtain list of genes, transcripts, splice sites ranked and filtered according to several criteria. Data can be viewed as tables, text files or through a simple genome browser which helps the visual inspection of the data. NGS-Trex is a simple tool for RNA-Seq data analysis mainly targeted to 'wet biology' researchers with limited bioinformatics skills."
According to the news editors, the research concluded: "It offers simple data mining tools to explore transcriptome profiles of samples investigated taking advantage of NGS technologies."
For more information on this research see: NGS-Trex: Next Generation Sequencing Transcriptome profile explorer. Bmc Bioinformatics, 2013;14 Suppl 7():S10. (BioMed Central - www.biomedcentral.com/; Bmc Bioinformatics - www.biomedcentral.com/bmcbioinformatics/)
The news editors report that additional information may be obtained by contacting I. Boria, Dipartimento di Chimica, Universita degli Studi di Milano, Milano, Italy. Additional authors for this research include L. Boatti, G. Pesole and F. Mignone.
Keywords for this news article include: Italy, Milano, Europe, Applied Bioinformatics.
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