By a News Reporter-Staff News Editor at Gene Therapy Weekly -- A new study on Biomaterials is now available. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Toxicity of polycations has been recognized since their first use in gene delivery. Bioreducible polycations attract attention because of their improved safety due to selective intracellular degradation by glutathione (GSH)."
Our news editors obtained a quote from the research from the Institute of High Energy Physics, "Here we present a systematic study of the toxicity of bioreducible poly(amido amine)s (PAA). PAA with increasing content of disulfide bonds were synthesized by Michael addition. Toxicity of PAA was evaluated in two cell lines with different innate levels of intracellular GSH. Increasing the content of disulfide bonds decreased the toxicity of PAA, with more significant decrease observed in cells with high GSH. Depleting intracellular GSH by diethyl maleate resulted in increased toxicity of bioreducible PAA. In contrast, increasing the GSH concentrations by growing cells in hypoxic conditions resulted in further decreased toxicity compared with cells grown in normoxic conditions. The presence of exofacial plasma membrane thiols selectively increased toxicity of bioreducible PAA while having no effect on non-degradable controls. These results improve our understanding of the cellular mechanisms of polycation toxicity."
According to the news editors, the research concluded: "They also shed light on the opposing effects of different cellular thiol pools on the toxicity of bioreducible polycations."
For more information on this research see: Opposing influence of intracellular and membrane thiols on the toxicity of reducible polycations. Biomaterials, 2013;34(34):8843-8850. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news editors report that additional information may be obtained by contacting C. Wu, Chinese Academy Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, People's Republic of China. Additional authors for this research include J. Li, Y. Zhu, J. Chen and D. Oupicky (see also Biomaterials).
Keywords for this news article include: Asia, Biotechnology, Beijing, Biomaterials, Gene Therapy, Bioengineering, People's Republic of China
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